Lm-LLO-Based Immunotherapies and HPV-Associated Disease

Figure 3

A schematic representation of the plasmid pActA-E7. The recombinant plasmid was used to transform the Lm strain XFL-7 to create Lm-ActA-E7. The vector includes a promoter (pHly) and signal sequence (ss) from the hly gene, the actA gene, the human papillomavirus 16 E7 gene, and the transcription factor prfA. XFL-7 is a prfA-deleted strain of Lm. Thus, only bacteria that retain the plasmid will replicate in vivo. Adopted and modified from [49].