Lm-LLO-Based Immunotherapies and HPV-Associated Disease
Figure 5
Lm-ActA-E7 induces E7-specific cytotoxic T-lymphocyte (CTL) activity. C57BL/6 mice were immunized with 0.1 median lethal dose of Lm-ActA-E7 or Lm-LLO-NP. A separate group of mice was left untreated. A booster immunization was administered 7 days later. Splenocytes were harvested 7 days after the booster and established in primary culture with irradiated TC1 cells for 7 days. Following the primary culture, CTL activity was assayed via chromium 51 (51Cr) release from EL4-E7 cells. The CTL activity was significantly higher in those mice that were vaccinated with Lm-ActA-E7 than in controls (). Results are expressed as the mean of triplicate cultures. These results are representative of 3 experiments. The figure and legend are adopted from [49].
