Cucurbitacin B Causes Increased Radiation Sensitivity of Human Breast Cancer Cells via G2/M Cell Cycle Arrest

Figure 5

Effect of cucurbitacin B on p21 protein expression in breast cancer cells. (a) Cells were treated with cucurbitacin B for 48 hr, and then the total proteins were extracted and performed western blotting to analyze 𝑝21 expression. Tubulin was used as an equal loading control. (b) Densitometric analyses of expression of 𝑝21 relative to the untreated control. ^∗𝑃<0.05 versus nontreated control, ^∗∗𝑃<0.01 versus nontreated control.

(a)

(b)