Signaling pathway involved in NNMT-mediated MMP-2 expression. (a) Various signaling pathway inhibitors were used to treat HEK293/NNMT cells transfected with full-length MMP-2 promoter plasmid and control reporter plasmid. The MMP-2 promoter activity was measured by luciferase reporter assay. Data are presented as the mean of triplicate replications. S.D. is indicated by error bars. ** versus untreated HEK293/NNMT cells. (b) HEK293/NNMT, 786O and Caki-1 cells were treated with LY294002 or AKT inhibitor IV for 18 h, and the expression of MMP-2 was measured by Q-PCR. Data are presented as the mean of triplicate replications. S.D. is indicated by error bars. *; ** versus untreated cells.
