Synergistic Effect of Garcinol and Curcumin on Antiproliferative and Apoptotic Activity in Pancreatic Cancer Cells
Figure 2
Garcinol-(upper panel) or curcumin-(lower panel) treated cytosolic extracts were used to evaluate induction of apoptosis in PaCa cells. (a) BxPC-3 and (b) Panc-1 using ELISA-Histone DNA Enrichment Assay. Results demonstrate a significant dose-dependent increase in apoptotic cells in individual treatment with either agent for 48 hours. Enrichment factor was measured using subtraction of background signal. * relative to control. Apoptotic morphological changes such as abnormal nuclear morphology, reduction in cell number with apoptotic body formation, and cell shrinkage were observed in a dose responsive manner on 48 hour treatment with (c) curcumin or (d) garcinol. BxPC-3 and Panc-1 cells were fixed with DAPI stain and visualized using fluorescence microscopy with an excitation maximum at 358 nm and emission maximum at 461 nm.
