Lack of Efficacy of Combined Antiangiogenic Therapies in Xenografted Human Melanoma
Figure 3
Dual immunofluorescence staining for CD31 and Tie2. (a) Tie2 was fluorescently labeled red using Cy3 while CD31 was fluorescently labeled green using FITC. Representative Tie2-positive blood vessels are marked with an asterisk while representative Tie2-negative blood vessels are labeled with arrows. (b) Quantification of average blood vessel densities (as number of CD31-positive vessels/mm2) between treatment groups. There were no statistically significant differences in blood vessel density between different treatment groups (). (c) Quantification of % Tie2-negative blood vessels in tumor xenografts treated with TekdeltaFc and/or LDM CTX. Graph depicts the average % of Tie2-negative blood vessels per treatment group; the difference between groups is not statistically significant ().
