Lack of Efficacy of Combined Antiangiogenic Therapies in Xenografted Human Melanoma
Figure 4
Immunostaining of blood vessels and pericytes in treated tumors. (a) Images show blood vessels immunofluorescently stained using antibodies to CD31 (Alexa 350; blue), desmin (Alexa 488; green), PDGFR-β (Cy3; red), and overlay of CD31/desmin/PDGFR-β. Asterisk marks blood vessel negative for mural cells (neither desmin nor PDGFR-β) while arrow indicates blood vessel with positive mural cell markers (desmin and PDGFR-β staining). (b) Quantification of blood vessel pericyte coverage in tumors treated with TekdeltaFc and/or LDM CTX. The percentage of “stable” blood vessels with pericyte coverage was significantly different between TekdeltaFc and all other treatment groups (*).
