Modulation of Telomeres in Alternative Lengthening of Telomeres Type I Like Human Cells by the Expression of Werner Protein and Telomerase
Figure 1
AG11395 does not support the replication of plasmids containing an SV40 origin of replication. (a) Three SV40-transformed cell lines were transfected with pRLSV40, which contains an SV40 origin of replication and pRLCMV, which does not contain an SV40 origin, in a 1 : 1 molar ratio along with pEGFP (as carrier DNA), or pBRSV40 (to supply wild-type T-antigen). Replication was measured by the ratio of pRLSV40 to pRLCMV as determined by qPCR, normalized to the ratio in the input DNA. AG11395 does not support replication of pRLSV40, even if wild-type T-antigen is provided by cotransfection. WI38 75.1 does support replication of pRLSV40, but only if wild-type T-antigen is provided by cotransfection. COS cells support efficient replication of pRLSV40, with or without additional T-antigen expression. (b) A short pulse (30 min) of EdU (5-ethynyl-2′-deoxyuridine) in AG11395 and WI38 75.1, S phase cell does not show a significant difference in the incorporation of EdU pattern in cells. Telomere pna FISH is (Cy3) red, EdU is (FITC) green, and DAPI-stained nuclei are blue.
