Modulation of Telomeres in Alternative Lengthening of Telomeres Type I Like Human Cells by the Expression of Werner Protein and Telomerase
Figure 2
WRN expression in AG11395 cells. (a) Western blot of WRN expression in AG11395 clones. 10 ug of total protein was prepared from the empty vector-infected and WRN expressing cell lines and blotted with the antibodies indicated. (b) WRN transgene expression in AG11395 was analyzed by indirect immunofluorescence (IF). WRN is present at increased concentration in the nucleoli of AG11395 cells expressing the WRN transgene. (c) Exogenous WRN expressed in AG11395 colocalizes with telomeres. PNA FISH and IF were performed with antibodies to WRN and TRF1; nuclei were visualized with DAPI. (d) Effect of WRN expression on telomeres in AG11395 cells. Telomere length increases after WRN expression in AG11395 cells. Quantitation of telomere length was performed by PNA FISH in the three AG11395 empty-vector and three WRN expressing clones.
