Modulation of Telomeres in Alternative Lengthening of Telomeres Type I Like Human Cells by the Expression of Werner Protein and Telomerase

Figure 4

APBs can be detected after WRN expression in AG11395 cells. APBs can be detected by colocalization of PML antigen with telomere markers. Indirect immunofluorescence to detect the PML protein was combined with PNA FISH to detect telomere DNA sequences. PML indirect immunofluorescence (green) and telomere PNA FISH (red) show significant colocalization in the ALT cell line, GM847. After re-expression of WRN protein, similar colocalization is observed in a subset of cells in AG11395 WRN protein expressing subline.