Modulation of Telomeres in Alternative Lengthening of Telomeres Type I Like Human Cells by the Expression of Werner Protein and Telomerase
Figure 5
Expression of hTERT in AG11395 modulates telomere length. (a) TRAP assay on hTERT-expressing cell lines. Exogenous expression of hTERT makes AG11395 cells TRAP positive. (b) Q-FISH of telomere repeat content in empty vector and mixed telomerase expressing AG11395 cells. Expression of telomerase increased the median telomere repeat signal in the population and caused loss of the telomeres with minimal detectable telomere repeat signal. (c) Q-PCR for SV40 sequences in the empty vector and hTERT expressing AG11395 sublines. SV40 sequences rapidly decreased by more than 60% after hTERT expression in AG11395. With continued passage, further loss of SV40 sequences was not observed.
