Research Article
Towards a “Sample-In, Answer-Out” Point-of-Care Platform for Nucleic Acid Extraction and Amplification: Using an HPV E6/E7 mRNA Model System
Figure 2
Automated LOC system for sample preconcentration, nucleic acid extraction, amplification, and real-time fluorescent detection. (a) Sample preparation chip: sample inlet [1], cell filter [2], a silica phase extraction filter [3], nucleic acid extraction reagent storage chamber [4A], storage chamber for DMSO and sorbitol [4B] turning valves [5], waste outlet [6], sample outlet [7], and a pressure sensor [8] (b) Sample preparation instrument (c) NASBA chip: sample inlet [1], supply channel [2], metering channels [3], valve 1 [4A], valve 2 [4B], valve 3 [4C], chamber 1 [5A], chamber 2 [5B], waste chamber [6], and instrument connection interface [7] (d) NASBA instrument.
