Towards a “Sample-In, Answer-Out” Point-of-Care Platform for Nucleic Acid Extraction and Amplification: Using an HPV E6/E7 mRNA Model System
Figure 3
(a) The amplification plot of the clinical sample 508 (HPV 33, 1 : 10 dilution). All the 7 reaction chambers filled with sample are classified as positive using our inhouse data analysis code also used in the conventional PreTect Analyser instrument. Reaction chamber number 8 was not filled with sample; hence, no increase of fluorescence was observed. The offset of the curve is also lower than the filled chambers, as the autofluorescence of the reaction mixture was not present. (b) Fluorescent micrograph of reaction chambers (8–1) after amplification of clinical sample 508 (HPV 33, 1 : 10 dilution). The air bubble located in reaction chamber number 4 contributes to the lower fluorescent signal detected by the NASBA instrument. No sample has entered reaction chamber 8.
