HAX1 Augments Cell Proliferation, Migration, Adhesion, and Invasion Induced by Urokinase-Type Plasminogen Activator Receptor

Figure 1

HAX1 colocalizes with uPAR upon stimulation of cells with uPA and uPA-ATF. (A) HEK293/uPAR and (B) MDA-MB-231 cells were transfected with pGEM-3Zf(+)∖HAX1, kept as negative control (a) or serum-starved overnight (b) and then treated with 100 ng/mL of either EGF (c), uPA, (d) or uPA-ATF (e) for 20 min. Cells were fixed and then immunostained with antibodies against uPAR (red) and HAX1 (green), and colocalization appeared as yellow colour. These cells were analysed using confocal laser scanning microscope and 60x oil immersion lens (final magnification 600x).