Research Article

Detection of Immunoglobulin G against E7 of Human Papillomavirus in Non-Small-Cell Lung Cancer

Figure 2

E7 antibody titrations and cut-off points of HPV-16 and/or -18 E7 positive. Scatter plot of ELISA values between HPV-16 and -18 E7s was used to decide positive and negative titrations. The threshold to be the positive was determined at the point where the slope of the predictive line generated by nonlinear optimization was changed. Each cut-off line for HPV-16 and -18 E7 positive was decided, and the patients who have higher titrations than the cut-off were selected as positives. Out of the positives, 5 were located above both cut-off lines, indicating cross-reactivity for both E7s. However, because 4 tended to get close to a cut-off line of one type, only one case (arrow) with the same distance to both cut-off lines was defined as the positive for both.
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