Enhancement of Cisplatin-Mediated Apoptosis in Ovarian Cancer Cells through Potentiating G2/M Arrest and p21 Upregulation by Combinatorial Epigallocatechin Gallate and Sulforaphane

Figure 3

EGCG and SFN combination treatment can induce apoptosis of ovarian cancer cells and enhance the apoptosis-induction effect of cisplatin. (A) and (C) Apoptosis induced by EGCG, SFN, and cisplatin treatment in A2780. (B) and (D) Apoptosis induced by EGCG, SFN and cisplatin treatment in A2780/CP20. Apoptosis cells include both early apoptosis and late apoptosis which are corresponding to right lower and upper quadrants. Dead cells correspond to the left upper quadrant. (a) control; (b) 10 μM EGCG; (c) 5 μM SFN; (d) 10 μM EGCG + 5 μM SFN; (e) 1.5 μM cisplatin for A2780, 8 μM cisplatin for A2780/CP20; (f) 10 μM EGCG + 5 μM SFN + 1.5 μM cisplatin for A2780, 10 μM EGCG + 5 μM SFN + 8 μM cisplatin for A2780/CP20. A2780 and A2780/CP20 were treated with EGCG or SFN for 3 days.