Enhancement of Cisplatin-Mediated Apoptosis in Ovarian Cancer Cells through Potentiating G2/M Arrest and p21 Upregulation by Combinatorial Epigallocatechin Gallate and Sulforaphane

Figure 5

EGCG and SFN combinational treatment can enhance p21 level induced by cisplatin in A2780 ovarian cancer cells. (a) Cisplatin can induce p21 protein expression in A2780 cells, while EGCG and SFN combinational treatment induces an even higher level of p21. (b) EGCG and SFN combination treatment can induce a higher level of p21 mRNA than either compound alone as evaluated by real-time PCR. (c) Expression level of p27 was not regulated by EGCG, SFN, or cisplatin treatment. All the experiments were repeated at least 3 times. ES: EGCG + SFN, Cis: cisplatin, ESCis: EGCG + SFN + cisplatin. ^∗𝑃<0.05, ^∗∗𝑃<0.01, ^∗∗∗𝑃<0.001.

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