AR-A 014418 Used against GSK3beta Downregulates Expression of hnRNPA1 and SF2/ASF Splicing Factors
Figure 4
U87 and U373 glioma cell lines were grown and plated day before, incubated with small molecule inhibitors against GSK3beta kinase (AR-A 014418, Manzamine A, and TDZ-8), Akt kinase inhibitor (LY294002 and Wortmanin), and Bortezomib (BR) for 24 hrs. (a) EGFR ligand dependent regulation of activating tyrosine phosphorylation of GSK3 kinase and downregulation of ERK1/2 pathway, after inhibiting the tyrosine phosphorylation of GSK3 kinase using AR-A 014418 for 24 hrs. (b) Differential localization of GSK3 kinase, beta catenin, and p-ERK1/2 was studied in cytoplasmic and nuclear fraction isolated from treated U373 glioma cell line. All experiments were repeated multiple times.
