Diverse Effects of Lysophosphatidic Acid Receptors on Ovarian Cancer Signaling Pathways

Figure 1

AKT and ERK expression is altered in LPAR OVCAR3 KO cells. (a) A representative photo of OVCAR3 and ES2 spheroids. (b)-(c) The p-AKT/AKT ratio is elevated in LPAR6KO cells in the 3D model () and decreased in the 2D model compared with control cells (). In addition, LPAR3KO cells cultured in 2D show decreased p-AKT levels (). Average ± SE; , , and compared with treated control (Student’s t-test). Group size: 3D untreated: control n = 14, LPAR2KO n = 13, LPAR3KO n = 14, LPAR6KO n = 16; treated: control n = 8, LPAR2KO n = 7, LPAR3KO n = 8, LPAR6KO n = 9; 2D untreated: control n = 3, LPAR2KO n = 5, LPAR3KO n = 4, LPAR6KO n = 4; treated: control n = 4, LPAR2KO n = 4, LPAR3KO n = 5, LPAR6KO n = 5.

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