Diverse Effects of Lysophosphatidic Acid Receptors on Ovarian Cancer Signaling Pathways

Figure 3

LPAR KO affects invasion. (a) To study the effect of ERK, cells were treated with 0.5 μM of the MEK inhibitor U0126 for 6 hours. The MEK inhibitor U0126 robustly reduced the invasiveness of OVCAR3 cells (). Average ± SE; (Student’s t-test). (b) To study the effect of LPA, cells were treated with 10 μM LPA for 6 hours. All three KO cells expressed a significantly lower invasive activity compared with controls. In LPAR3KO cells, treatment with LPA decreased invasion (group size: n = 4 for each group), whereas in LPAR2KO and LPAR6KO cells, there was no statistically different effect (). Average ± SE; , , and compared with treated control and compared with untreated KO (Student’s t-test). (c) Effect of LPARs on invasive activity in ES2 cells. All three KOs decreased invasion in ES2 cells. Cells were treated with 10 μM LPA for 6 hours. LPA had a significant positive effect on invasion in ES2 control and LPAR6KO cells compared with their untreated cells. Average ± SE; , compared with the same untreated group.

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