High Aldehyde Dehydrogenase Levels Are Detectable in the Serum of Patients with Lung Cancer and May Be Exploited as Screening Biomarkers

Figure 2

Western blot analysis for the polyclonal anti-RTNKEASI serum in cancer cell lines and cellular supernatant. (a) Protein extracts from non-small cell lung cancer A549, normal lung tissue MRC-5, Burkitt lymphoma CA46, leukemia HL60, breast cancer MCF-7, cervical carcinoma Hela, and prostate cancer PC-3 cell lines were loaded into SDS gel and subjected to electrophoresis and immunoblotting with the polyclonal anti-RTNKEASI serum from Rockland. High 55 kDa protein levels were identified in A549 cells and a lower amount in Hela, PC-3, CA46 and HL-60 cell lines whereas no protein levels were observed in MCF-7 cell line from breast cancer and in MRC-5 from normal lung tissue. An anti-ß-actin antibody was used for a loading control. (b) Protein extracts from cellular supernatant of non-small cell lung cancer A549 and cell lysate from A549 and MRC-5 cells were loaded into SDS gel and subjected to electrophoresis and immunoblotting with the polyclonal anti-RTNKEASI serum from Rockland. A specific band at 55 kDa was detected in the cellular supernatant sample with respect to cell lysate from A549; MRC-5 was used as negative control.

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