Oral Administration of Semicarbazide Limits Weight Gain together with Inhibition of Fat Deposition and of Primary Amine Oxidase Activity in Adipose Tissue
Table 2
Gene expression in visceral adipocytes from semicarbazide-drinking or control mice.
Gene
Control
Semicarbazide-treated
Amine oxidases:
MAO-A
66.2 ± 22.3
35.9 ± 11.7
MAO-B
154 ± 21
133 ± 31
AOC2
2.0 ± 0.4
1.5 ± 0.2
AOC3
4750 ± 555
2420 ± 275**
Adipokines:
Adiponectin
22568 ± 1942
19068 ± 2195
Resistin
3837 ± 300
4143 ± 374
leptin
2068 ± 365
853 ± 80**
IL-6
792 ± 230
718 ± 198
TNF-alpha
16.9 ± 2.6
23.5 ± 8.1
Apelin
26.0 ± 9.3
12.5 ± 4.3
Fuel metabolism:
GLUT1
187 ± 28
103 ± 18*
GLUT4
440 ± 38
429 ± 51
LPL
47742 ± 3860
39274 ± 3152
mRNA abundance of the indicated genes was determined by rt-PCR and expressed using the arbitrary units detailed in Section 2. Mean ± SEM of 8 determinations. Different from control at ; .
