476240.fig.004a
476240.fig.004b
476240.fig.004c
476240.fig.004d
Figure 4: Duolink proximity ligation assay to detect interactions between PTPIP51 and the insulin receptor (IR), respectively PTPIP51 and PKA in standard diet animals. (A) Interaction between PTPIP51 and the IR. (B) Interaction between PTPIP51 and PKA. (C) Negative control, PTPIP51 and the IR in testis, no interaction known. (D) Negative control, DPLA without primary antibodies in adipose tissue. Nuclei are marked by DAPI staining. Arrows: positive reactions. Bars (A, B): 50 μm. Bars (C, D): 20 μm.