Complement Receptors C5aR and C5L2 Are Associated with Metabolic Profile, Sex Hormones, and Liver Enzymes in Obese Women Pre- and Postbariatric Surgery
Figure 3
Association of hepatic C5L2/C5aR mRNA ratio with menopausal status and metabolic profile. mRNA was extracted from liver biopsies and reverse-transcribed to cDNA and gene expression was evaluated by RT-PCR with the indicated primers with all results expressed relative to GAPDH using the method as described in detail in Section 2. (a) Comparison of hepatic C5L2/C5aR mRNA expression ratio and plasma ASP levels in premenopausal and postmenopausal groups. ASP values were log transformed for statistical analysis. ((b)–(d)) Linear plot correlation of liver enzymes ALT, AST, and GGT with C5L2/C5aR mRNA ratio. (e) Linear plot correlation of ApoB and C5L2/C5aR mRNA ratio. (f) Comparison of preoperative BMI (kg/m2) and fasting liver enzymes (IU/L) (left -axis) and ApoA1/B ratio (right -axis) before bariatric surgery in subjects separated based on low versus high C5L2/C5aR mRNA ratio, compared by unpaired Student’s -test.
