Prevalence of GCKR rs1260326 Variant in Subjects with Obesity Associated NAFLD and T2DM: A Case-Control Study in South Punjab, Pakistan

Figure 1

Tetra-arms PCR and gel electrophoresis of GCKR rs1260326 variant. Tetra-arms PCR was optimized and analyzed by gel electrophoresis. DNA was extracted from all samples and amplified with GCKR rs1260326 specific primers. This figure shows 1 to 10 representative samples from NAFLD subjects. The 541 bp amplicon was considered enteral control for all PCR reactions. The 338 bp amplicon was identified as a homozygous mutant C/C, while 240 bp was considered a homozygous wild type T/T. Tetra-arm primer amplified 338 bp in mutant variants and 240 bp in wild-variants and both amplicons in heterozygous variants. Amplicon length was identified with a 100 bp DNA ladder (L).