Gestational Early-Time Restricted Feeding Results in Sex-Specific Glucose Intolerance in Adult Male Mice

Figure 2

Early life body composition, food intake, and glycemic homeostasis. (a) Body weight in grams from PND21–PND70 in males and females, averaged by age, maternal-feeding regimen, and sex. (b) Fat mass in grams from PND21–PND70 in males and females, averaged by age, maternal-feeding regimen, and sex. (c) Lean mass in grams from PND21–PND70 in males and females, averaged by age, maternal-feeding regimen, and sex. (d) Food intake in kcals per mouse per day, averaged by week, maternal-feeding regimen, and sex. value <0.05 for diet. (e) Insulin tolerance test (ITT)∼PND 70, averaged by maternal-feeding regimen, sex, and time in minutes. (f) Area under the curve (AUC) for ITT, averaged by maternal-feeding regimen and sex. value <0.05 for the effect of diet in males. (g) Glucose tolerance test (GTT)∼PNG 70, averaged by maternal-feeding regimen, sex, and time in minutes. (h) AUC for GTT, averaged by maternal-feeding regimen and sex. value <0.05 for the effect of diet in males. (i) Fasting blood glucose (FBG) PND 70, averaged by maternal-feeding regimen and sex. Animals included in body composition measurements, FBG, ITT, and GTT: n = 11 eTRF males, 16 AL males, 19 eTRF females, and 17 AL females. Number of cages in food intake analysis: n = 4 eTRF males, 5 AL males, 4 eTRF females, and 5 AL females.