Gestational Early-Time Restricted Feeding Results in Sex-Specific Glucose Intolerance in Adult Male Mice

Figure 3

Body composition, food intake, and glycemic response to high-fat, high-sucrose diet feeding in adulthood. (a) Body weight in grams from PND 70 to 175 in males and females, averaged by age, maternal-feeding regimen, and sex. (b) Fat mass in grams from PND 70 to 175 in males and females, averaged by age, maternal-feeding regimen, and sex. (c) Lean mass in grams from PND 70 to 175 in males and females, averaged by age, maternal-feeding regimen, and sex. (d) High-fat, high-sucrose diet (HFHS) intake in kcals per mouse per day averaged by week, maternal-feeding regimen, and sex. (e) Insulin tolerance test (ITT) after 10 week of HFHS, averaged by age, maternal-feeding regimen, sex, and time in minutes. (f) Area under the curve (AUC) for insulin tolerance test, averaged by maternal-feeding regimen and sex. value <0.05 for diet in males. (g) Glucose tolerance test (GTT) after 10 weeks of HFHS, averaged by maternal-feeding regimen, sex, and time in minutes. (h) Area under the curve (AUC) for GTT after 10 weeks of HFHS, averaged by maternal feeding regimen and sex. value <0.05 for the effect of diet in males. (i) Fasting blood glucose (FBG) after 10 weeks HFHS, averaged by maternal-feeding regimen and sex. (j) Glucose-stimulated insulin secretion (GSIS), averaged by maternal-feeding regimen, sex, and time. (k) Fold change of insulin secretion during GSIS, averaged by maternal-feeding regimen and sex. value <0.05 for the effect of sex. Animals included in body composition, FBG, ITT, GTT, and GSIS: n = 11 eTRF males, 16 AL males, 19 eTRF females, and 17 AL females. Cages in food intake analysis: n = 4 eTRF males, 5 AL males, 4 eTRF females, and 5 AL females.