The Concentrations of Interleukin-6, Insulin, and Glucagon in the Context of Obesity and Type 2 Diabetes and Single Nucleotide Polymorphisms in IL6 and INS Genes

Table 2

The conditions for PCR and restriction enzyme digestion.

SNP	Primers	PCR-RFLP conditions
rs3842729	Forward primer-5′ GGC TTG ACC GGC CAG GGT GTC CCC 3′; Reverse primer-5′ ACC CCC AGC TGC AAC CTC AGG GGC T 3′	The initial denaturation-95°C for 5 min, denaturation-95°C for 40 s, annealing-67.4°C for 35 s, elongation-72°C for 25 s, the final elongation72°C for 10 min
rs1800795	Forward primer-5′ TGA CTT CAG CTT TAC TCT TTG T 3′; Reverse primer-5′ CTG ATT GGA AAC CTT ATT AG 3′	The initial denaturation-95°C for 15 min, denaturation-95°C for 40 s, annealing-55°C for 35 s, elongation-72°C for 40 s, the final elongation-72°C for 10 min
SNP	Restriction enzymes	Restriction enzyme digestion conditions
rs3842729	MspI	37°C for 1 hour
rs1800795	SfaNI	37°C for 16 hours