274317.fig.002a
(a)
274317.fig.002b
(b)
274317.fig.002c
(c)
274317.fig.002d
(d)
Figure 2: Glo I expression and activity in transgenic mouse lenses. (a) H&E staining of Wt and Glo I transgenic lenses showed no morphological changes in 6-month old animals. No difference was noticeable between the Wt and transgenic lens. (b) Immunohistochemistry using a monoclonal antibody for human Glo I showed human Glo I expression in epithelial cells and outer cortical fiber cells. Glo I immunoreactivity was absent in Wt lenses. (c) In situ hybridization shows Glo I mRNA in outer cortical and epithelial cells in Glo I transgenic animals. (d) Glo I activity was measured in the water-soluble lens proteins. Glo I-catalyzed formation of S-D-lactoylglutathione from MGO and GSH was monitored at 240 nm. The data are the mean ± SD from 4 lenses.