465824.fig.001a
(a)
465824.fig.001b
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465824.fig.001c
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465824.fig.001d
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465824.fig.001e
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465824.fig.001f
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Figure 1: (a) Representative Western Blot showing increased pERK5 and total ERK5 after 24 hrs HG treatment which subsequently decreased after 48 hrs. (b): Densitometric quantification of pERK5 expression. (c–f): Real-time PCR showed that following incubation in HG, ERK5, MEF2C and KLF2 mRNA expression were increased after 24 hrs and then were decreased after 48 hrs. On the other hand, VEGF mRNA expression did not increase until after 48 hrs of HG treatment. LG:5mmol/l D-glucose; OC: osmotic control, 25 mmol/l L-glucose; HG: 25 mmol/l D-glucose. [Data in “B” are expressed as a ratio to β-actin. mRNAs are expressed as a ratio to 18S, normalized to controls, * significantly different from LG, n=3-6/group.]