NADPH Oxidase versus Mitochondria-Derived ROS in Glucose-Induced Apoptosis of Pericytes in Early Diabetic Retinopathy
Figure 1
Retinal capillary pericytes express NADPH oxidase. Aliquots of total cellular protein were separated by SDS-PAGE, electrophoretically transferred onto Hybond-ECL membranes. Blots were probed with (a) anti-gp91phox antibody and anti-p47phox antibody. Protein antibody complexes were detected using secondary antibody conjugated with horseradish peroxidase and the ECL detection system. Lane 1: BRP, lane 2: BREC (bovine retinal capillary endothelial cells), Lane 3: HUVEC (human umbilical vein endothelial cells), and lane 4: U937 cells (human leukocytes). (b) Representative Western blots of gp91phox, p47phox and tubulin. (c) Expression of gp91phox protein expression in BRP exposed to high glucose (HG, 25 mM) for 48 h. Densitometric ratio (intensity gp91phox immunoreactive band/intensity of the tubulin immunoreactive band) is expressed as % of normal glucose (NG, 5.6 mM). (d) Expression of p47phox protein expression in BRP exposed to high glucose (HG, 25 mM) for 48 h. Densitometric ratio (intensity of p47phox immunoreactive band/intensity of the tubulin immunoreactive band) is expressed as % of normal glucose (NG, 5.6 mM). Data are represented as Means SEM of 3-4 separate experiments. versus control.
