NADPH Oxidase versus Mitochondria-Derived ROS in Glucose-Induced Apoptosis of Pericytes in Early Diabetic Retinopathy

Figure 6

Apocynin reverses glucose-induced CML formation in BRP. Subconfluent cells in 24-well plate were exposed to normal glucose (NG, 5.6 mM) and continuous high glucose (HG, 25 mM) and in the presence of 500 $\mu$M apocynin (HGA) or 1 $\mu$M MitoQ (HGMQ) for 4 days. After incubation, the cells were washed twice with ice-cold PBS, lysed and CML were detected using competitive ELISA. Data are presented as Mean $\pm$ SEM of 8–13 samples. ${}_{}{}^{*}P<.05$ versus NG, ${}_{}{}^{**}P<.05$ versus HG.