NADPH Oxidase versus Mitochondria-Derived ROS in Glucose-Induced Apoptosis of Pericytes in Early Diabetic Retinopathy
Figure 7
Exposure to high glucose increases intracellular glucose concentration. Subconfluent cells in 24-well plate were exposed to normal glucose (NG, 5.6 mM) and high glucose (HG, 25 mM) and in the presence of 500 M apocynin (NGA, HGA) or 1 M MitoQ (NGMQ, HGMQ) for 4 days. The cells were washed twice with ice-cold PBS, lysed and intracellular glucose concentrations were measured using the Amplex Red glucose assay kit (Molecular Probe). Data are Means ± SEM of 5–14 separate experiments. versus NG; versus HG.
