Efficient Transduction of Feline Neural Progenitor Cells for Delivery of Glial Cell Line-Derived Neurotrophic Factor Using a Feline Immunodeficiency Virus-Based Lentiviral Construct

Figure 1

GDNF-transduced cNPCs: morphology and reporter gene expression. Feline NPCs transduced using a bicistronic lenti-GDNF-GFP vector and cultured under proliferation conditions (UM) for 60 days (p9–p26). Cellular growth, morphology, and GFP expression were monitored over this time period. In this figure, paired phase contrast ((a), (c), (e), (g), (i), (k)) and fluorescence ((b), (d), (f), (h), (j), (l)) micrographs of the same field are presented for each of 6 sequential time points, as indicated. Transduced cNPCs exhibited consistent mophologies, continued growth, and sustained GFP expression throughout the period examined. Bars = 100 𝜇m.