Efficient Transduction of Feline Neural Progenitor Cells for Delivery of Glial Cell Line-Derived Neurotrophic Factor Using a Feline Immunodeficiency Virus-Based Lentiviral Construct

Figure 2

Growth properties of transduced versus nontransduced cNPCs. The growth of lenti-GDNF-GFP vector transduced cNPCs was compared to nontransduced cNPCs under proliferation conditions (UM). One flask of each type of cells was harvested and counted daily for 3 consecutive days. From this data it can be seen that the transduced cNPCs continued to proliferate despite overexpression of GDNF and that growth was similar to that of nontransduced cells out to day 2, after which the nontransduced cells exhibited relatively greater growth at the day 3 time point.