Efficient Transduction of Feline Neural Progenitor Cells for Delivery of Glial Cell Line-Derived Neurotrophic Factor Using a Feline Immunodeficiency Virus-Based Lentiviral Construct

Figure 3

Flow cytometric analysis of GFP expression after induction of differentiation. Nontransduced cNPCs and lenti-GDNF-GFP vector transduced cNPCs cultured under proliferation conditions (UM) were compared to transduced cNPCs cultured for 10 days in Ultraculture-based medium without EGF or bFGF and containing 10% FBS in order to induce differentiation (UM-FBS). Curve A: nontransduced cNPCs as negative controls; curve B: lenti-GDNF-GFP transduced cNPCs and curve C: lenti-GDNF-GFP transduced cNPCs in UM-FBS. Induction of differentiation did not attenuate expression of the GFP reporter gene.