Efficient Transduction of Feline Neural Progenitor Cells for Delivery of Glial Cell Line-Derived Neurotrophic Factor Using  a Feline Immunodeficiency Virus-Based Lentiviral Construct

<table>Expression profiles of cNPCs before and after transduction. The relative impact of GDNF overexpression on transcript expression levels was evaluated using qPCR analyses for a profile of 32 genes, which included <svg height="16.6625" id="M29" style="vertical-align:-3.56265pt" version="1.1" viewbox="0 0 10 16.6625" width="10" xmlns="http://www.w3.org/2000/svg" xmlns:xlink="http://www.w3.org/1999/xlink">
<g transform="matrix(.017,-0,0,-.017,.062,12.162)"><path d="M558 587q0 -32 -14 -61t-40 -53.5t-48.5 -41t-54.5 -36.5q144 -51 144 -174q0 -55 -43.5 -108t-104.5 -87q-77 -42 -131 -42q-31 0 -54 20t-31 47l11 18q48 -29 108 -29q79 0 119.5 43t40.5 109t-44.5 107.5t-119.5 50.5l22 47q34 1 65 21q96 61 96 157q0 42 -24 67.5
t-62 25.5q-24 0 -43.5 -9t-35 -29.5t-27 -44t-22.5 -63t-19.5 -75.5t-18.5 -91q-57 -294 -68 -380q-26 -190 -35 -200q-26 -31 -97 -37l-4 26q19 9 48 170l77 413q23 121 52.5 187.5t83.5 114.5q70 62 148 62q51 0 88.5 -34t37.5 -91z" id="x1D6FD"></path></g>
</svg>-actin as a housekeeping gene. Lenti-GDNF-GFP vector transduced cNPCp20 cells were compared to nontransduced cNPCp20 cells (with nontransduced cells set to 1.00). GDNF transcript level was over 14,000-fold higher in transduced versus nontransduced cells (note that <svg height="11.175" id="M30" style="vertical-align:-0.0pt" version="1.1" viewbox="0 0 11.6625 11.175" width="11.6625" xmlns="http://www.w3.org/2000/svg" xmlns:xlink="http://www.w3.org/1999/xlink">
<g transform="matrix(.017,-0,0,-.017,.062,11.113)"><path d="M667 650l-9 -28q-53 -5 -76 -17t-64 -59q-51 -61 -175 -225q-21 -29 -27 -55l-27 -136q-13 -65 -0.5 -80t83.5 -22l-7 -28h-280l8 28q64 4 81 19t30 83l25 128q6 35 -7 65l-98 231q-17 41 -32.5 52t-68.5 16l8 28h252l-6 -28l-40 -4q-27 -3 -33 -12.5t2 -31.5
q8 -26 43 -107.5t61 -134.5q114 145 174 240q14 24 8 33t-37 13l-34 4l8 28h238z" id="x1D44C"></path></g>
</svg>-axis has break to accommodate value). The value for GDNF was vastly greater than any other changes in transcript level across the profile examined.</table>

Journal of Ophthalmology

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Figure 6

Figure 6: Efficient Transduction of Feline Neural Progenitor Cells for Delivery of Glial Cell Line-Derived Neurotrophic Factor Using  a Feline Immunodeficiency Virus-Based Lentiviral Construct 

Figure 6 | Efficient Transduction of Feline Neural Progenitor Cells for Delivery of Glial Cell Line-Derived Neurotrophic Factor Using  a Feline Immunodeficiency Virus-Based Lentiviral Construct 