Variables and Strategies in Development of Therapeutic Post-Transcriptional Gene Silencing Agents
Figure 8
Allelic variant human opsin construct. The hhRz cleavage site at V230 (GUC) was mutated by site-specific mutagenesis to the degenerate human valine codon GUG. The V230V human opsin cDNA in a CMV expression vector (pCDNA3) was expressed in HEK293S cells along with control human WT opsin CMV expression vector. Immunocytochemistry with 1D4 opsin monoclonal and an FITC-labeled secondary antibody was conducted. Abundant human WT opsin expression and cell surface trafficking was noted in cells expressing WT (a) or V230V aWT proteins (b).
