Vascular bed of a human retina imaged by second harmonic generation (SHG). Serial -sections, spaced 12 μm apart, of a human retina are shown beginning with (a) through (f). The images shown are collected using the 800 nm near infrared laser excitation with a collection window of 390–410 nm. The collagen structure of a large blood vessel is clearly visible through the series, which represents a height of 60 μm.
