Essential Role of Thioredoxin 2 in Mitigating Oxidative Stress in Retinal Epithelial Cells
Figure 2
Expression profile of Trx1 and Trx2 in ARPE cells. ARPE cells were infected with AAV-Trx1 or AAV-Trx2, and Trx-overexpressing cells were established (ARPE-Trx1, ARPE-Trx2). The expression of Trx1 and Trx2 is indicated by the fluorescence of the reporter protein pmCherry, which was fused to Trx. Localization of Trx2 to mitochondria was examined by the fluorescence of a mitochondrial marker dye. ARPE cells infected with AAV-pmCherry served as a control. Fluorescence was observed using microscope (a) and confocal microscope (b).
