Correlations between ERG, OCT, and Anatomical Findings in the rd10 Mouse
Figure 2
sd-OCT of wild type and rd10 mice. (a) Set-up. The mouse is positioned in front of the magnifying lens of the OCT on the chin rest of equipment usually used for application in humans. ((b)–(h)) OCT images of wild type and rd10 mice at different ages. All images are taken at the height of the papilla and displayed with the pigment epithelium to the top. Images of rd10 mice are printed in black on a white background (first column) as well as in white on a black background (second column). Red arrows in (b) (first column) demonstrate the evaluation of thickness at the height of the papilla by six averaged cuts through the retina. Red bars in ((c)–(f)) (first column) mark the outer nuclear layer (ONL). In (g) (first and second column) red stars indicate a separation of the retina from the pigment epithelium. Separation was only observed in vivo in two animals of nine and 24 weeks of age, respectively (g). (i) Evaluation of retinal thickness in wild type and rd10 mouse in the OCT. Values obtained from six cuts through the retina at the height of the papilla were averaged and plotted against the age (each age group: , both eyes per ). Thickness of the whole retina is illustrated in ((i)1) (= wild type) and ((i)2) (= rd10 mouse). ((i)3): thickness of ONL (outer nuclear layer, somata) + inner and outer segments of the photoreceptors (IS + OS). In ((i)4), retinal thickness except photoreceptor layers is illustrated (GC = ganglion cell layer, IPL = inner plexiform layer, INL = inner nuclear layer, and OPL = outer plexiform layer). Values represent mean ± SD ((i)1) (wild type) for animals at the age of 2 weeks: **** versus animals older than 5 weeks; ((i)2) (rd10) for animals of 2 and 3 weeks **** versus animals older than 5 weeks; ((i)3) (rd10) for animals of 2 weeks **** versus animals older than 5 weeks; one-way ANOVA with Bonferroni’s post hoc test).
