Correlations between ERG, OCT, and Anatomical Findings in the rd10 Mouse
Figure 5
Immunohistochemical staining of wild type (a) and rd10 retina at 7 weeks (b) and at 24 weeks (c). Staining 1: recoverin (all photoreceptors and type 2 bipolar cells) in green; HCN1 (photoreceptor somata and inner segments, processes in IPL, red); rhodopsin (rod outer segments, blue; puncta in the inner retina represent blood vessels labeled unspecifically by the secondary antibody). The ONL in the rd10 mouse is considerably reduced to only one row of somata at 7 weeks (b) and completely lost at 24 weeks (c). Staining 2: rod bipolar cells (PKCα, green), horizontal cells (anti-28 kDa calcium-binding protein = CabP, red), and amacrine cells (calretinin, blue). Insets show processes of rod bipolar and horizontal cells at higher magnification. In rd10 retina, such processes are lost. Staining 3: astrocytes (anti-GFAP, green), Müller cells (anti-glutamine synthetase = GlutS, red), and cone outer and inner segments (PNA, blue). In rd10 mice, Müller cell processes are also GFAP-positive. Scale bar represents 50 μm in overviews and 10 μm in insets. Bar diagram in (d) illustrates retinal thickness evaluated in vivo by OCT ( per age group; both eyes per ) in comparison to measurements performed in immunohistochemistry (; both eyes per ). Thickness values in vivo and in vitro differ by 10 to 15%. Values represent mean ± SD.
