The Polymorphisms with Cataract Susceptibility Impair the EPHA2 Receptor Stability and Its Cytoprotective Function

Figure 5

The nonsynonymous polymorphisms nullify the antioxidative capacity of EPHA2. (a-b) The lipid oxidation was evaluated with MDA assays in the HLECs before and after H₂O₂ treatment. The overexpression of EPHA2 reduces the absorbance value of cell lysate with H₂O₂ treatment, while the introduction of or mutant does not decline the lipid oxidation. (c-d) The SOD activity and the total antioxidative capacity were tested with SOD and TAC assay kit, respectively. The data are presented as U/mg or μmol/mg proteins. The upregulation of SOD level and total antioxidant content by EPHA2 is abrogated by the cataract-associated SNPs. , , and versus vector control; , compared with wild-type EPHA2.

(a)

(b)

(c)

(d)