Journal of Ophthalmology

Research Article

Proteomic Study of Retinal Proteins Associated with Transcorneal Electric Stimulation in Rats

Table 1

Differentially expressed proteins by proteomics from the retinas with/without TES. K-152 to K-210 represent ID number of spots and sequence coverage, and the theoretical values of pI and Mr were obtained from the Profound search. The calculations of the experimental pI and Mr were based on the migration of the protein on a 2D gel. The expression changes present in the rat retinal protein volume ratio after 50 mA, 100 mA, and 200 mA currents of TES for 30 minutes and 24 hours after TES. “o” in expression means that the expression was undetectable in the PD-Quest-based analyses.


Number	Protein	Protein ID	Gene ID	Probability		Sequence coverage (%)	Matched peptide	Mes. peptide	pI	MW (kDa)	Suspected pI	Suspected MW (kDa)	Ratio of protein volume after TES
													30 min			24 hrs
													50	100	200	50	100	200

K-152	Guanine nucleotide binding protein	NP_112298.1	gi 13591957		2.2	27	8	56	5.5	42.41	2–4	32–44	0	1.26 ± 0.86	0	0	0.91 ± 0.32	0.71 ± 0.59
K-154	Calcium-binding and coiled coil domain 1	NP_631929.1	gi 21070934		2.43	8	5	64	4.8	77.77	3–4.5	44–92	0.34 ± 0.59	0	0	0.34 ± 0.59	2.01 ± 2.47	0.6 ± 0.37
K-157	Keratin 80	NP_001008815.1	gi 57012388		0.9	14	6	50	5.9	51.05	3–5	44–92	0.77 ± 0.23	0.67 ± 0.29	2.58 ± 2.06	1.17 ± 0.15	1.04 ± 0.27	1.15 ± 0.26
K-158	Epidermal growth factor receptor	NP_113695.1	gi 25742617		1.21	7	7	20	6.7	138.3	3–5.5	92–200	0	0	0	0	0.83 ± 0.14	0
K-168	Dynamin-like protein DLP1 isoform DLP1-37	AAD31278.1	gi 4868358		0.59	19	10	49	7	80.79	4–6	32–92	0	0	0.84 ± 0.21	0	0	0
K-172	Adenylate cyclase 10	NP_067716.1	gi 11067413		0.4	10	12	54	6.6	188.29	4–6.5	67–140	0	0	0	0	1.31 ± 0.56	0
K-173	Rab GDI beta/GDI-2	CAA52412.1	gi 396433		0.87	18	5	22	5.7	51.18	4–6.5	44–92	0.99 ± 0.17	0.41 ± 0.54	0.66 ± 1.14	0	0	1.36 ± 0.83
K-175	Prohibitin	NP_114039.1	gi 13937353		2.12	32	8	61	5.6	29.86	4.5–7	24–44	0	0.65 ± 0.32	1.03 ± 0.53	0.78 ± 0.7	0	0.96 ± 0.95
K-183	SIAH-2/seven in absentia 2	AF389477_1	gi 19550385		0.57	13	3	21	6.3	29.47	6–8.5	32–67	0	0.58 ± 0.51	0	0.33 ± 0.34	0.33 ± 0.57	0
K-189	Immunoglobulin heavy chain 1a	P20761.1	gi 121055		1.14	19	5	28	8	37.11	8–11	24–44	0	0	0	0	0	1 ± 0.43
K-190	Serine protein inhibitor/SERPIN	AAL99574.1	gi 19850068		1.23	36	10	88	9.4	47.57	3.5–5.5	16–32	0	0	0	0	2.28 ± 1.97	1.17 ± 0.26
K-191	Transforming growth factor bets regulator 4	AAH87073.1	gi 56541114		1.04	16	9	70	8.9	71.62	8.5–10.5	32–67	0	0	0	0.41 ± 0.53	0.04 ± 0.04	0
K-192	Vimentin	NP_112402.1	gi 14389299		1.9	16	11	65	5.1	53.77	4–6	44–92	0	0	1 ± 0.52	0	0	0
K-193	Angiopoietin-like 3	AAH88192.1	gi 56971793		0.84	28	6	46	6.1	37.14	6.5–8.5	32–67	0	0	1 ± 0.73	0	0	0
K-196	Regulator of calcineurin-2	NP_783168.1	gi 28212228		0.65	9	2	13	5.4	27.64	4–6	24–44	0	0	0	0	0	1 ± 1.12
K-197	Ras-related GTP binding B	AAH78760.1	gi 50927585		2.43	15	5	49	6.1	43.61	5.5–8	24–44	0	0	0	0	0	1 ± 0.89
K-199	Ca²⁺/Mg²⁺ ATPase	AAA57270.1	gi 602486		1.36	11	4	43	5.5	57.01	3.5–5.5	44–92	0	0	0	0	0.79 ± 0.18	1.63 ± 1.28
K-200	Vimentin-type intermediate filament	NP_001001720.1	gi 48843733		1.66	30	3	16	6.4	18.88	4–6	0–24	0	0	2.8 ± 1.64	0	0.35 ± 0.57	0.77 ± 0.63
K-201	Tenascin-X	AAA91987.1	gi 841426		0.7	16	3	30	6.4	23.71	4.5–6.5	16–32	0	0	0	0	0.13 ± 0.63	1.48 ± 0.48
K-202	Hydroxyphenylpyruvate dioxygenase/F alloantigen	AAA40740.1	gi 202924		1.63	15	4	32	6.3	43.6	4.5–7	32–44	0	0.54 ± 0.42	0	0.69 ± 0.3	0	0.33 ± 0.58
K-203	Elk protein	CAA31777	gi 56095		0.73	6.6	2	25	6.6	43.05	4.5–7	24–44	0	1 ± 0.69	0	0	0	0
K-205	Rho-GTPase activating protein 24	AAH85797.1	gi 55249719		1.66	15	9	58	8.7	73.98	5–7	32–67	1.04 ± 0.29	0.58 ± 0.39	0.74 ± 0.4	0.34 ± 0.59	0	0
K-207	Ankyrin	AAB47551.1	gi 1841966		1.35	20	10	84	8.2	90.36	5–7	44–92	0	1 ± 0.13	0	0	0	0
K-209	Dihydroxyacetone phosphate acryltransferase	NP_445862.1	gi 16758146		1.09	31	14	106	8.2	77.74	6–9	32–67	0	0	0	1 ± 0.35	0	0
K-210	Lymphocyte cytosolic protein 1	AAH83855.1	gi 54035327		1.34	27	11	89	5.2	70.73	6.5–8.5	44–67	0	0	1.04 ± 0.88	0.71 ± 0.62	1.08 ± 0.85	0