Journal of Ophthalmology / 2015 / Article / Tab 1 / Research Article
Proteomic Study of Retinal Proteins Associated with Transcorneal Electric Stimulation in Rats Table 1 Differentially expressed proteins by proteomics from the retinas with/without TES. K-152 to K-210 represent ID number of spots and sequence coverage, and the theoretical values of pI and Mr were obtained from the Profound search. The calculations of the experimental pI and Mr were based on the migration of the protein on a 2D gel. The expression changes present in the rat retinal protein volume ratio after 50 mA, 100 mA, and 200 mA currents of TES for 30 minutes and 24 hours after TES. “o” in expression means that the expression was undetectable in the PD-Quest-based analyses.
Number Protein Protein ID Gene ID Probability Sequence coverage (%) Matched peptide
Mes. peptide pI MW (kDa) Suspected pI Suspected MW (kDa) Ratio of protein volume after TES 30 min 24 hrs 50 100 200 50 100 200 K-152 Guanine nucleotide binding protein NP_112298.1 gi 13591957 2.2 27 8 56 5.5 42.41 2–4 32–44 0 1.26 ± 0.86 0 0 0.91 ± 0.32 0.71 ± 0.59 K-154 Calcium-binding and coiled coil domain 1 NP_631929.1 gi 21070934 2.43 8 5 64 4.8 77.77 3–4.5 44–92 0.34 ± 0.59 0 0 0.34 ± 0.59 2.01 ± 2.47 0.6 ± 0.37 K-157 Keratin 80 NP_001008815.1 gi 57012388 0.9 14 6 50 5.9 51.05 3–5 44–92 0.77 ± 0.23 0.67 ± 0.29 2.58 ± 2.06 1.17 ± 0.15 1.04 ± 0.27 1.15 ± 0.26 K-158 Epidermal growth factor receptor NP_113695.1 gi 25742617 1.21 7 7 20 6.7 138.3 3–5.5 92–200 0 0 0 0 0.83 ± 0.14 0 K-168 Dynamin-like protein DLP1 isoform DLP1-37 AAD31278.1 gi 4868358 0.59 19 10 49 7 80.79 4–6 32–92 0 0 0.84 ± 0.21 0 0 0 K-172 Adenylate cyclase 10 NP_067716.1 gi 11067413 0.4 10 12 54 6.6 188.29 4–6.5 67–140 0 0 0 0 1.31 ± 0.56 0 K-173 Rab GDI beta/GDI-2 CAA52412.1 gi 396433 0.87 18 5 22 5.7 51.18 4–6.5 44–92 0.99 ± 0.17 0.41 ± 0.54 0.66 ± 1.14 0 0 1.36 ± 0.83 K-175 Prohibitin NP_114039.1 gi 13937353 2.12 32 8 61 5.6 29.86 4.5–7 24–44 0 0.65 ± 0.32 1.03 ± 0.53 0.78 ± 0.7 0 0.96 ± 0.95 K-183 SIAH-2/seven in absentia 2 AF389477_1 gi 19550385 0.57 13 3 21 6.3 29.47 6–8.5 32–67 0 0.58 ± 0.51 0 0.33 ± 0.34 0.33 ± 0.57 0 K-189 Immunoglobulin heavy chain 1a P20761.1 gi 121055 1.14 19 5 28 8 37.11 8–11 24–44 0 0 0 0 0 1 ± 0.43 K-190 Serine protein inhibitor/SERPIN AAL99574.1 gi 19850068 1.23 36 10 88 9.4 47.57 3.5–5.5 16–32 0 0 0 0 2.28 ± 1.97 1.17 ± 0.26 K-191 Transforming growth factor bets regulator 4 AAH87073.1 gi 56541114 1.04 16 9 70 8.9 71.62 8.5–10.5 32–67 0 0 0 0.41 ± 0.53 0.04 ± 0.04 0 K-192 Vimentin NP_112402.1 gi 14389299 1.9 16 11 65 5.1 53.77 4–6 44–92 0 0 1 ± 0.52 0 0 0 K-193 Angiopoietin-like 3 AAH88192.1 gi 56971793 0.84 28 6 46 6.1 37.14 6.5–8.5 32–67 0 0 1 ± 0.73 0 0 0 K-196 Regulator of calcineurin-2 NP_783168.1 gi 28212228 0.65 9 2 13 5.4 27.64 4–6 24–44 0 0 0 0 0 1 ± 1.12 K-197 Ras-related GTP binding B AAH78760.1 gi 50927585 2.43 15 5 49 6.1 43.61 5.5–8 24–44 0 0 0 0 0 1 ± 0.89 K-199 Ca2+ /Mg2+ ATPase AAA57270.1 gi 602486 1.36 11 4 43 5.5 57.01 3.5–5.5 44–92 0 0 0 0 0.79 ± 0.18 1.63 ± 1.28 K-200 Vimentin-type intermediate filament NP_001001720.1 gi 48843733 1.66 30 3 16 6.4 18.88 4–6 0–24 0 0 2.8 ± 1.64 0 0.35 ± 0.57 0.77 ± 0.63 K-201 Tenascin-X AAA91987.1 gi 841426 0.7 16 3 30 6.4 23.71 4.5–6.5 16–32 0 0 0 0 0.13 ± 0.63 1.48 ± 0.48 K-202 Hydroxyphenylpyruvate dioxygenase/F alloantigen AAA40740.1 gi 202924 1.63 15 4 32 6.3 43.6 4.5–7 32–44 0 0.54 ± 0.42 0 0.69 ± 0.3 0 0.33 ± 0.58 K-203 Elk protein CAA31777 gi 56095 0.73 6.6 2 25 6.6 43.05 4.5–7 24–44 0 1 ± 0.69 0 0 0 0 K-205 Rho-GTPase activating protein 24 AAH85797.1 gi 55249719 1.66 15 9 58 8.7 73.98 5–7 32–67 1.04 ± 0.29 0.58 ± 0.39 0.74 ± 0.4 0.34 ± 0.59 0 0 K-207 Ankyrin AAB47551.1 gi 1841966 1.35 20 10 84 8.2 90.36 5–7 44–92 0 1 ± 0.13 0 0 0 0 K-209 Dihydroxyacetone phosphate acryltransferase NP_445862.1 gi 16758146 1.09 31 14 106 8.2 77.74 6–9 32–67 0 0 0 1 ± 0.35 0 0 K-210 Lymphocyte cytosolic protein 1 AAH83855.1 gi 54035327 1.34 27 11 89 5.2 70.73 6.5–8.5 44–67 0 0 1.04 ± 0.88 0.71 ± 0.62 1.08 ± 0.85 0