Expression of HGF and c-Met Proteins in Human Keratoconus Corneas

Table 3

Primary antibodies and negative controls used for immunostaining.

Primary antibody	Company	Final concentration used
Mouse anti-human HGF	Santa Cruz Biotechnology, Inc. (Dallas, Texas, USA)	2 µg/mL
Rabbit anti-human c-Met	Santa Cruz Biotechnology, Inc.	0.4 µg/mL
Mouse anti-human IgG	Zymed Laboratories (Thermo Fisher Scientific, Waltham, Massachusetts, USA)	2 µg/mL
Rabbit anti-human IgG	Zymed Laboratories	0.4 µg/mL
Alexa Fluor 488 goat anti-mouse IgG	Molecular Probes (Life Technologies, Carlsbad, California, USA)	2 µg/mL
Alexa Fluor 488 donkey anti-rabbit IgG	Molecular Probes	2 µg/mL
Alexa Fluor 594 donkey anti-rabbit IgG	Molecular Probes	2 µg/mL
Hoechst	Molecular Probes	1 µg/mL