iTRAQ-Based Proteomic Analysis of Visual Cycle-Associated Proteins in RPE of rd12 Mice before and after RPE65 Gene Delivery

Figure 2

Identification and quantification of differentially expressed proteins by iTRAQ. The RALBP-1, RBP-1, and IRBP were identified as differentially expressed visual cycle-associated proteins among the untreated rd12, treated rd12, and normal control C57BL/6J eyes. RALBP-1, RBP-1, and IRBP were remarkably reduced in the untreated rd12 mice (<0.7×) compared to those of C57BL/6J sample. In the treated rd12 eyes, RALBP-1 was increased to 6-fold of the untreated rd12 level, although it was still lower than the normal level (0.66-fold); RBP-1 was increased to 2-fold of the untreated rd12 level and 1.50-fold of the normal level. IRBP level was still lower in the treated rd12 eyes.