Properties of Retinal Precursor Cells Grown on Vertically Aligned Multiwalled Carbon Nanotubes Generated for the Modification of Retinal Implant-Embedded Microelectrode Arrays

Figure 3

Effects of direct contact on viability of L-929 cells. Cells were plated on a glass substrate and on the blank, MWCNT-Fe, MWCNT-Fe-Pt, and MWCNT-Fe-Ti wafer slices ( experiment), stained using FDA/PI, and counted 72 hours after seeding. (a) Fluorescence microscopy allowed for differentiation between vital (green) and dead (red) cells (scale bar: 100 μm). (b) The total cell numbers were normalized to the respective glass control. (c) The quantity of dead cells is shown as a percentage of the corresponding total cell number. For each substrate, 6 randomly selected microscopic fields were analyzed. Data are represented as mean ± SD. They were compared to the glass control and analyzed using unpaired two-tailed test, showing no significant differences.

(a)

(b)

(c)