Expression of Wnt/β-Catenin Signaling Pathway and Its Regulatory Role in Type I Collagen with TGF-β1 in Scleral Fibroblasts from an Experimentally Induced Myopia Guinea Pig Model

Figure 3

Primary culture and identification of scleral fibroblasts in guinea pig. (a) At the third day, a small amount of fibroblasts climbed out from the scleral tissue. (b) At the sixth days, there were about 60% of fibroblasts. (c) At the fifth days at the second passage, cell fusion was about 80%–90%. (d) Cultured scleral fibroblasts were stained positive for vimentin. (e) In blank control group, the staining in cultured scleral fibroblasts was negative. (f) Cultured scleral fibroblasts were stained negative for keratin (original 200, bar = 50 µm).

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