SENP1-Mediated Desumoylation of DBC1 Inhibits Apoptosis Induced by High Glucose in Bovine Retinal Pericytes

Figure 6

Sumoylation of DBC1 contributed to SENP1-mediated apoptosis under high glucose conditions. (a) After HEK293T cells were transfected with Flag-DBC1-WT or Flag-DBC1-K591R, with or without RH-SUMO3 as indicated for 48 h, cell lysates were subjected to immunoprecipitation with anti-Flag antibody followed by immunoblot analysis. (b) Effect of siRNA-mediated knockdown on DBC1 expression in BRPCs incubated in normal glucose. (c) Analysis of cellular apoptosis levels in the BRPC treatment groups for 48 h: NG, NG+Flag-DBC1-WT, NG+Flag-DBC1-K591R, HG, HG+Flag-DBC1-WT, and HG+Flag-DBC1-K591R. (d) Cleaved PARP and BCL-2 protein expression profiles in the six groups. Data are presented as their means ± SEM. Bars indicate SDs. versus HG+Flag-DBC1 WT.